PERSPECTIVAS MÉDICAS


Perspect. Med. ISSN 0100-2929

Basic and Clinical Research

Perspectivas Médicas, 25(3): 21-30, set./dez. 2014.
DOI: 10.6006/perspectmed.20140303.0265557174

 

Pregabalin protects neurons of pro-inflammatory action of the T lymphocytes encephalitogenic

 
Keywords: labor, obstetric; analgesia; clonidine; fentanyl; anesthesia, epidural
 

Authors:

Gustavo Ferreira Simões¹,²,³,?, Rodrigo Fabrizzio Inácio¹, Giuliano Roberto Gonçalves¹, Taize Machado Augusto4, Rodolfo Thomé¹, Liana Verinaud¹, Alexandre Leite Rodrigues de Oiveira¹, Fábio Lima Leite³

¹ Universidade Estadual de Campinas – UNICAMP – Campinas, São Paulo, Brasil
² Faculdade de Ciências Médicas da Santa Casa de São Paulo – FCMSCSP – São Paulo, São Paulo, Brasil
³ Grupo de Pesquisa em Nanoneurobiofísica (GNN), Departamento de Física, Química e Matemática (DFQM), Universidade Federal de São Carlos – UFSCar – Campus de Sorocaba, São Paulo, Brasil
4 Faculdade de Medicina de Jundiaí – FMJ – Jundiaí, São Paulo, Brasil

 

Correspondence to:

Gustavo Ferreira Simões

Laboratório de Regeneração Nervosa, Instituto de Biologia, Universidade Estadual de Campinas – UNICAMP, Rua Monteiro Lobato, 255, CEP: 13083-970, Campinas, SP, Brasil. Phone: 55 19 3521-6646e-mail: gfsimoes2@gmail.com

 
ABSTRACT

Multiple sclerosis is an autoimmune disease of the CNS (Central Nervous System) with progressive character. The experimental autoimmune encephalomyelitis (EAE) model is able to reproduce the pathogenicity of multiple sclerosis-associated immune response. The encephalitogenic T lymphocytes play an important role in multiple sclerosis and have been shown to modulate these cells through therapeutic strategies. Pregabalin is a Gamma-Amino Butyric Acid (GABA) analog drug that acts as an anticonvulsant which reduces the release of norepinephrine and glutamate, and have the ability to modulate the excitatory synaptic transmission and promote anti-apoptotic and anti-inflammatory effects. OBJECTIVES: Morphologically analyze in vitro neuroprotection promoted by pregabalin in neuronal cells cultured with conditioned medium from encephalitogenic T lymphocytes. METHODS: C57BL/6 females six weeks old were EAE induced. After ten days from EAE induction, the animals were sacrificed and the spleens collected aseptically for preparation of cell suspensions, which were T lymphocytes enriched. Culture supernatants were collected and used for preparation of conditioned medium from encephalitogenic T lymphocytes. Neuronal cells were cultured and then treated with conditioned medium and pregabalin at 30, 150 and 300 µg/ml and subsequently processed for immunocytochemistry (synapsin antiserum). RESULTS: Neuronal cells treated with conditioned medium alone showed reduction in total cell number 24 hours after initiation of treatment. After treatment with pregabalin we observed neuroprotection, as evidenced by the total number of cells and increased immunoreactivity for synapsin. CONCLUSION: Pregabalin is a drug with potential neuroprotective effects during the course of EAE.

 

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